I made some progress today on the Arduino side of things. So I have a working prototype now.
This is exciting news. I read your last post a few days ago and started typing up a response but ran out of time and never finished it. I don’t know what temperature-resistance curve Aquacomputer products are calibrated for. I suspect their products are designed to work with B3950 thermistors, but Aquacomputer does not specify a Beta value, so measurements would have to be made to confirm. Most companies that sell temp sensors for computer cooling loops just say they are 10K NTC thermistors, but they don’t provide the Beta number.
The DS3502 has 128 steps (0-127) with a linear resistance range of approximately 79Ω (step 0) to 10kΩ (step 127). To emulate temperatures between 20°C and 60°C using a B3950K thermistor curve, you'd need a resistance range of 12.47KΩ to 2.47KΩ. The DS3502's 10kΩ maximum means it cannot reach the 12.47kΩ needed for 20°C emulation without additional external resistors. I thought the DS1841 would be a better choice, but it has a fatal flaw. It's a logarithmic digi-pot (256 positions) that better matches the exponential resistance curve of a thermistor. It also has a built-in 70-point Look Up Table (LUT) specifically designed for thermistor emulation, covering -40°C to 100°C. Instead of direct wiper control, you can use Temperature LUT Mode - send a temperature value, and the chip sets the appropriate resistance. There's also a Custom LUT mode for programming your own temperature-resistance curve. The problem - The DS1841 spec says its resistance range is 3.7kΩ to 22kΩ, and its temperature range is from -40°C to 100°C. If this is correct, the DS1841 is not using a B3950K temperature-resistance curve, so the LUT table would not have the correct values for the Quadro if the Quadro calibration is based on a B3950K thermistor. Even if you programmed a custom LUT, the device still cannot go below 3.7KΩ (49°C in the B3950 curve).
If you are going to stay with the DS3502, I think if you add a 2.2KΩ resistor in series with it (in rheostat mode), it will give you the required range to emulate from 20°C and 60°C using a B3950K thermistor curve. The problem is its a 128-step device. I did some digging but so far have not found an ideal digi-pot that has 256 steps, operates in the required range, and comes in an easy to work with package. The Microchip MCP41U83 is a 10-bit device available in several variants. I think the 10KΩ model would work well with a 2.2KΩ 1% resistor in series (each step is ~9.8Ω), but it is a TSSOP package that is very small and would be difficult to work with. The Microchip MCP4131-103E/P (10KΩ, 256 step, SPI, DIP package) with a 2.2KΩ 1% resistor would be easier to work with, but its SPI (4-wire) instead of I2C (2-wire). The best option I found so far is the Microchip MCP4018-203E/MS. It is 20KΩ so covers the required range, but it is 128-step. It is I2C and comes in a SOIC-8 package, so not too hard to work with. The temperature accuracy should be ~0.33°C at 20°C and ~1.73°C at 60°C, which is better than a 10K NTC thermistor. For an MCU, I would use an Espressif ESP32-S3 or a Raspberry PI RP2040 PICO. An UNO is fine for testing, but it's kind of big to put in a computer case. I will keep looking when I have time.