No, only a USB connaction is possible. You can use the aquabus only with a aquaero 5/6.
Thank you for the response

If both the Quadro and D5 Next are connected to a internal USB 2.0 plug they will still both communicate to AquaSuite, correct? And all 4 channels of the Quadro can be controlled independently?
My motherboard only has 1x USB header so I was hoping the Quadro and D5 Next could be connected through Aquabus.
Have you considered the
HUBBY7? It should solve your single USB port problem.
After posting and continuing to evaluate options I did find the option of a 2.0 splitter. There are little 1 into 2 plugs but I think a Hubby7 or the NZXT module would be best as they have PSU power. The NZXT has an extra two USB-A too which would be nice to plug my keyboard and mouse into and save I/O ports for other stuff.
With that option it would still be a very clean minimal setup: (Quadro controlling fans/RGB)(D5 Next pump with Temp/Flow display) I really like the Next since it has the digital readout for Temp/Flow and I don't have to figure out any other displays connected to sensors. I also don't think I'll fully utilize the Aquaero since I really only need the fans controlled and the pump. This may be the perfect setup.