you need to connect by usb 1st for the device to be recognised by the pc so having a hubby helps if you have a lot to connect. you can disconnect after and use just aquabus. however I kept my usb connected so future updates are easy to do so in answer to your question yes you can have either or have both
I have Aquaero 6 xt, 1x hubby7, 1 x poweradjust 3, an mps 400 and 4x splitty 9, 1 of which is setup as aquabus hub. the Aquaero, hubby7, mps400 and the poweradjust are all connected by both usb and aquabus.